Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
Mutations are caused by changes in nucleotide bases. these altered base formed different amino acid depending upon nucleotide base sequence that code specific amino acid. ... even though some mutations, can have a more effect on amino acid coding, which can affect what kind of proteins are produced.
