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sesenic [268]
3 years ago
11

If we have a plate that has 2500 colonies on it, can we use this plate to calculate CFU's

Biology
1 answer:
AleksAgata [21]3 years ago
6 0

No, the solution with bacteria in it needs to be diluted in order to be in the range of 30 – 300.

A colony-forming unit –CFU is used to estimate the number of viable (ability to divide by binary fission) microbes in a sample.  

A sample of microbes is diluted and spread on a plate. Then, the number of colonies is counted, assuming that each colony have grown from a single colony-forming unit, or CFU.

Plates with more than 300 colonies are difficult to count while those with less than 30 colonies give statistically unreliable numbers of colonies to count.

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If we follow the pyramid of tropic level known as " Energy level" Heat is lost to the environment at each of the trophic levels of the ecosystem. The producer which has large area of the pyramid has a large energy, and as the primary consumer(second tropical level) which are herbivores feed on the producer(first tropical level)grass , it only takes just like 10% energy from the producer and the secondary consumer ( third tropical level) herbivores feed on primary consumer, and only get away with 10% of the energy and as the trend goes up the "Energy keep decreasing inform of heat" like that.

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