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Tju [1.3M]
3 years ago
14

Can somebody help me out with this question because I’m really confused??

Biology
1 answer:
Arada [10]3 years ago
8 0

Answer:

i believe its b

Explanation:

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A DNA fragment is introduced into the lacZ gene of a plasmid, which also contains an ampicillin resistance gene. What is the app
zhenek [66]

Answer: The bacteria transformed with this particular plasmid will form white colonies on the plates containing ampicillin and Xgal.

Explanation: The lacZ gene produces an enzyme called β-galactosidase which is responsible for the breakdown of lactose into glucose and galactose. The lacZ gene is one of the three genes (the other two being lacA and lacY) of the lac operon which is responsible for the transport and mechanism of lactose in E. coli and many other bacteria.

In recombinant DNA technology, when a plasmid is to be used to transform a host cell, such markers are used to help screen the transformed cells from the ones that have not taken up the plasmid. Xgal present in the plates is an artificial substrate which is hydrolyzed by

β-galactosidase into 5-bromo-4-chloro-indoxyl which will dimerize and oxidise into 5,5'-dibromo-4,4'dichloro-indigo. This is a blue pigment which will give blue color to the bacterial cells. Introducing a DNA fragment in this lacZ gene will make it non-functional so it will not be able to produce the enzyme.

Therefore, when a bacterial cell is transformed with a plasmid containing ampicillin resistance gene and a DNA fragment introduced in the lacZ gene and then grown on plates containing ampicillin and Xgal, white colored colonies will appear. The white colonies will show the bacterial cells that have successfully taken up the plasmid with the DNA fragment incorporated in the lacZ gene as this will render the gene non-functional and will not produce β-galactosidase which will breakdown Xgal to give blue colonies. Since the plates contain ampicillin, only the bacterial cells that have been successfully transformed with the plasmid ( the ones that have the DNA fragment and the ones without it) will grow as the ampicillin resistance will give them resistance against ampicillin in the plates. The bacterial cells that have not taken up the plasmid will not be resistant to ampicillin and will not form colonies on the plate.

This is called blue-white screening which is used to identify successfully transformed host cells. A picture of this is given in the attachment, taken from the following website:

https://www.mun.ca/biology/scarr/Blue_&_White_Colonies.html

8 0
3 years ago
What is the difference between Parasympathetic nervous system vs sympathetic nervous system
svp [43]

Answer: sympathetic nervous system prepares body for intense physical activity and known as flight or fight response whereas parasympathetic nervous system is almost the opposite, it relaxes the body and slows many human functions

Explanation:

7 0
3 years ago
And like someone gets cancer from UV rays, can it be passed down to their children
Fofino [41]
In some cases, skin cancers are hereditary and an increased risk of developing the disease can be passed from parent to child. If members of the same or multiple members being diagnosed the chance it being passed down to your children is higher.

Hope this helps:)
3 0
1 year ago
Read 2 more answers
All of the following are positive aspects of PACs EXCEPT?
muminat
I think the answer is C 
6 0
2 years ago
Read 2 more answers
If a meal supplies 110 g of carbohydrates, 25 g of protein, 20 g of fat, and the equivalent of 5 g of alcohol, what % of the kca
mars1129 [50]

Answer:

0.058 % kcalories comes from carbohydrate.

Explanation:

1 g of carbohydrtes gives 4 calories, 1 g of protein gives 4 kcalories, 1 gm of fat gives 9 calories and 1 g of alcohol gives 7 calories of energy.

Thus in a meal  that contains 110 g carbohydrates gives 110 x 4 = 440 calories,  25 g protein gives 25 x 4 = 100 calories, 20 g fat gives 20 x 9 = 180 calories and 5 g alcohol gives 5 x 7 =  35 calories.

Total calories in a meal  440+100+180+35 = 755.

Therefore % of carbogydrate will be 440x 100 divided by 755 = 58.27 %

In kcalories % would be 58.27/1000 = 0.5827 %  

5 0
3 years ago
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