Phenol red is used as the indicator for the Urea Medium because the indicator detects the formation of ammonia caused by the hydrolysis of urea done by the bacteria. Bromothymol blue is used in Simmons Citrate Agar because it effectively indicates the formation of carbonates formed by the consumption of citrate.
Answer:
C. The enzyme changes shape and is no longer able to bind the substrate.
Explanation:
The change in heat changed the size of the enzyme. The enzyme changed size and shape which made it incapable of bonding with the substrate.
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