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katovenus [111]
3 years ago
5

What are two main types of posttranscriptional modifications that take place in the mrna of eukaryotes?

Biology
1 answer:
erastovalidia [21]3 years ago
4 0

Answer:

Addition of poly A tail and and addition of 5' cap

Explanation:

In eukaryotes, many post-transcriptional modification takes place like 5' capping, polyadenylation, and splicing. In 5' capping 7-methyl guanosine is added toward the 5' end of mRNA by the action of a enzyme called guanyl transferase. This cap protects mRNA from degradation.

In polyadenylation up to 250 adenosine nucleotides are added at the 3' end of the mRNA and it is called poly-A tail. Poly A polymerase is the enzyme that is responsible for polyadenylation.  

Therefore two main post transcription modifications are 5' capping and polyadenylation.

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You have a 15kb plasmid. EcoRI cuts the plasmid into 7kb and 8kb pieces, and BamHI cuts the plasmid into 1kb and 14kb pieces. If
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Answer:

a. Bands at 1kb, 6kb and 8kb

Explanation:

The EcoRI and BamHI are the restriction enzymes which cut the DNA sequence especially a plasmid at specific sites called the restriction sites.

The restriction enzymes produces bands of specific length therefore these restriction enzymes are used to estimate the approximate length of the DNA.

In the given question, the

1. EcoRI- produces two strands of 7 kb and 8 kb

2. BamHI- produces two strands of 1kb and 14kb

This shows that the length of DNA sequence is 15kb

But when the DNA strand are digested with both the enzymes simultaneously then it will produce three bands as:

i) 14 kb can be broken down in 2 bands of 6 kb and 8 kb

ii) 1 kb band is already produced by the Bam HI.

This shows that 1+6+8= 15 kb

Thus, Option-A is correct.

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