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Vinil7 [7]
3 years ago
12

An amino acid mixture consisting of alanine, phenylalanine, and lysine is to be separated by normal phase HPLC. The stationary p

hase is polar and the mobile phase is a nonpolar solvent. The first amino acid to be eluted will be_______ . The last amino acid to be eluted will be:_______
Biology
1 answer:
Lera25 [3.4K]3 years ago
7 0

Answer: Phenylanine; Lysine

An amino acid mixture consisting of alanine, phenylalanine, and lysine is to be separated by normal phase HPLC. The stationary phase is polar and the mobile phase is a nonpolar solvent. The first amino acid to be eluted will be Phenylanine. The last amino acid to be eluted will be: Lysine

Explanation:

In normal phase HPLC (High performance liquid chromatography), the polar stationary phase would retain the less nonpolar amino acids (alanine and lysine) while the nonpolar mobile phase would permit the quick elution of the aromatic amino acid, phenylanine. Thus, phenylanine is first eluted.

This is then followed by the elution of the amino acid with nonpolar side chain, alanine. Thus, alanine is the second to be eluted.

Lastly, the positively charged amino acid, lysine will be released by the stationary phase. Thus, lysine is the last to be eluted

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HELP!! WILL INCLUDE LINK
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First of all, I want to say that I was literally learning about this stuff not 10 minutes ago. That is a re.tardedly creepy coincidence.

1.) Science is used mostly through the field of forensics in order to study crime scenes, collect data like fingerprints and hair fibers to try to identify the suspect, as well as use things like blood splatter to try to recreate what happened during the crime. this data is then used as evidence, either for or against a suspect, in order to sway the jury's opinion.

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4.) In a criminal trial, a suspect or defendant is being accused of a crime. It is up to the prosecutor to prove that the defendant is guilty of that crime. It is up to the defense attorney to defend the defendant and prove that he is not guilty. It is up to the judge to listen to all the evidence and afterwards to give his verdict. The scientists can be used by either the prosecutor or defense attorney to bring forth evidence that can support either of their sides.

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