Small bumps on endoplasmic reticulum are the ribosomes which give a rough appearance to the endoplasmic reticulum and therefore called Rough endoplasmic reticulum. These ribosomes on RER are required for protein synthesis. Protein is synthesised on ribosomes by translation and transported by endoplasmic reticulum to the cell
If there was any air conditioning making the bubbles go in different directions. If you used more solution than you did with other. Bubbles if you didn’t blow the same amount of times for each bubble. If you didn’t blow with the same air flow