1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Andrews [41]
4 years ago
12

Describe each step required to make a gene knock-out in mice.

Biology
1 answer:
Ahat [919]4 years ago
6 0

Answer:

1. Designing of the target vector.

2. Insertion of the target vector into the embryonic stem cell

3. Selection of the cells

4. Injecting selected cells into the new cells

5. Breeding of mouse with a normal mouse

Explanation:

The target vectors are made by inserting markers into them. The target vector is inserted into ES cells. The vector generally recombined with the target gene and knock-out that gene. Sometimes, recombination of vectors occurs at incorrect place.

The cells survive in the presence of markers only if the target gene is incorporated with target vector. The cells with the target vector are injected into a normal developing embryo of mouse.

The developed mouse will consist of heterozygous knockout cells and its own cells. The normal mouse is bred with this chimeric mouse. The offspring of these mouse are able to transfer knock-out gene to next generation.

You might be interested in
What structures covers and protects the gills
GaryK [48]

An operculum covers and protects the gills.

Explanation:

An operculum is a flexible bony type of plate which protects the sensitive gills. When water is inhaled through the mouth it moves over the gills and is exhaled from below the operculum ( gill cover). gills remove oxygen that is present in the water and diffuse carbon dioxide from fish's body. Some fishes have spines which are located on the opiculum( gill cover) which acts as a defense mechanism to secure them from the predators.

7 0
4 years ago
in order to provide meaningful information measurements must be expressed in terms of their A. Numbers B. Units C. Accuracy D. Q
shepuryov [24]
I would go for C. Accuracy is the best one if you have the right information then your numbers should be right
4 0
3 years ago
Read 2 more answers
Describe each of the following: <br> Sickle Cell Anemia <br> Diabetes <br> Acne <br> HELP!!
sergejj [24]

Answer:

1. Sickle cell anemia is an inherited red blood cell disorder in which there aren't enough healthy red blood cells to carry oxygen throughout your body. Normally, the flexible, round red blood cells move easily through blood vessels. In sickle cell anemia, the red blood are shaped like sickles or crescent moons.

Treatments: Blood transfusion

Symptoms: Pain

2. Diabetes is the <u>condition in which the body does not properly process food for use as energy</u>. Most of the food we eat is turned into glucose, or sugar, for our bodies to use for energy. The pancreas, an organ that lies near the stomach, makes a hormone called insulin to help glucose get into the cells of our bodies.

3. Acne is a skin condition that occurs when your hair follicles become plugged with oil and dead skin cells. It causes whiteheads, blackheads or pimples. Acne is most common among teenagers, though it affects people of all ages.

3 0
3 years ago
Read 2 more answers
Both liver cells and lens cells have the genes for making the proteins albumin and crystalline. However, only liver cells expres
Pani-rosa [81]

Answer: The claim that gene regulation results in differential gene expression and influences cellular products (albumin or crystalline) is best supported by t<u>he specific transcription factors made in a cell.</u>

Explanation:

Genes store deoxyribonucleic acid or DNA that makes up the genome. The genetic information within genes is expressed through a process called transcription followed by translation.

During this, the genotype of the organism is changed into its phenotype or physical characteristics genes are controlled or regulated through certain molecular mechanisms   These essentially determine transcription and translation and whether gene products is active or in active genes are turned on when they are able to transcribe the genome.  

gene expression may be regulated   via

  • modulation before or after the transcription process
  • modulating translation
  • modulation after translation

A control for the transcription process  is called a transcription factor. These are DNA sequence specific proteins that are capable of activating or suppressing transcription at certain sites. In different cell types, different combinations of transcription factors contribute to gene expression.

7 0
4 years ago
Which type of biome is characterized by the following descriptions? moderate temperatures with seasonal changes very high amount
4vir4ik [10]
The answer is Tropical Rainforest Biome

The tropical rainforest has a moderate temperature with seasonal changes. The amount of rainfall in a tropical rainforest is high. It has a thick canopy of trees which filters out a majority of the sunlight which is why the forest floor is almost shaded. 
5 0
3 years ago
Read 2 more answers
Other questions:
  • Betty has cervical cancer. it is probably related to the ________ infection she got back in college.
    7·1 answer
  • What are 2 nutrients of concern to the Chesapeake Bay?
    15·1 answer
  • What is the best description of all subjects studied in earth science ?
    7·1 answer
  • Definition for SISTER CHROMATID.
    9·1 answer
  • ⚠️HELP⚠️
    14·1 answer
  • Which of the following explains why plant cells must also perform cellular respiration?
    7·1 answer
  • How many trophic levels are there in the following food web?
    15·1 answer
  • How to determine if two species are different species
    13·1 answer
  • Which of these functions of life refers to<br> maintenance of an internal balance?
    11·1 answer
  • Kingdom Protista or Protoctista<br>Helpp :(​
    5·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!