Answer:
Explanation:
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Answer:in culture plates cell density of the edges is often more than the center of the dish,
what causes this distribution?
Explanation:
Answer:
Transcription and translation occur simultaneously in prokaryotes.
Explanation:
Prokaryotes lack membrane-bound organelles and therefore, do not have a nucleus. Transcription and translation occur in the cytoplasm. As soon as the required length of mRNA is being formed, ribosomes join it and start the process of translation. Prokaryotic genes lack introns. The primary transcript formed by transcription in prokaryotes does not undergo splicing.
On the other hand, the process of transcription occurs in the nucleus in eukaryotes while translation occurs in the cytoplasm. The primary transcript formed by transcription in eukaryotes undergoes modifications to remove introns and to add a poly-A tail and 5' cap. Post-transcriptional modifications and spatial separation of two processes in eukaryotes result in slower translation than prokaryotes.
Answer:
e. None of the above
Explanation:
For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.
i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.
