Answer:
4. c. Deletion/ frameshift mutation
5. a. Tyr Tyr Asp Ser Gly
6. A frameshift occurred which altered the identity of the amino acids following the initiator 6. methionine, but not the length of protein Q.
Explanation:
4)
A single base pair at the first position of the Arg codon i.e. CGA has been deleted. Due to the deletion, the reading frame has shifted one nucleotide backwards, altering the amino acid sequence of the protein.
5)
The genomic DNA sequence is 5'-ATG ATA CTA AGG CCC-3'. Therefore, the complementary mRNA will be 5'-UAC UAU GAU UCC GGG-3'. According to the genetic code, this mRNA sequence encodes the following amino acid sequence: Tyr Tyr Asp Ser Gly
6)
The wild type DNA sequence is:
5'-ACUGUAAUGGAGUCAGAUGAUGCAUUAAGGAGAAAUUGAAAUAAA-3'
The mutant i.e. mRNA with the added G will be
5'
ACUGUAAUGGGAGUCAGAUGAUGCAUUAAGGAGAAAUUGAAAUAAA-3'
This is a single base pair insertion and frameshift mutation that shifts the reading frame one base pair forwards. The codon that was originally GAG coding for glutamic acid (Glu) gets converted to GGA that codes for glycine. Therefore, the amino acid sequence has been altered but the polypeptide chain will still have 13 amino acids.
Answer:
A. NADH and FADH2 both donate electrons at the same location.
Explanation:
In the respiratory chain, four large protein complexes inserted into the mitochondrial inner membrane transport NADH and FADH₂ electrons (formed in glycolysis and the Krebs cycle) to oxygen gas, reducing them to NAD⁺ and FAD, respectively.
These electrons have great affinity for oxygen gas and, when combined with it, reduce it to water molecules at the end of the reaction.
Oxygen gas effectively participates in cellular respiration at this stage, so its absence would imply interruption of the process.
NADH and FADH₂ electrons, when attracted to oxygen, travel a path through protein complexes, releasing energy in this process.
The energy released by the NADH and FADH₂ electrons in the respiratory chain in theory yields <u>34</u> <u>ATP</u>, however, under normal conditions an average of 26 ATP molecules is formed.
If we consider that these 26 molecules are added to the two ATP formed in glycolysis and two ATP formed in the Krebs cycle, it can be said that cellular respiration reaches a maximum yield of 30 ATP per glucose molecule, although theoretically this number was 38 ATP per glucose molecule.
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