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2 years ago

The ________ are a diverse group of organic substances including triglycerides, phospholipids, and the sterols.

Biology

1 answer:

andrew11 [14]2 years ago

3 0

The lipids are a diverse group of organic substances including triglycerides,phosphlipids and the sterols

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Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes

s2008m [1.1K]

Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.

Following test and control samples will be used for the assay.

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

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2 years ago

What three conditions must a fossil meet in order to be an index fossil?

Darya [45]

It must be widespread, limited in geological time, and distinctive.

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2 years ago

What is a major difference between the structure of a cell wall and a cell membrane

poizon [28]

Answer:

The cell wall is the outside layer and the cell membrane is the inner layer. Cell walls are made of cellulose and lignin, while cell membranes contain phospholipids. Cell walls are made of cellulose and cell membranes are made of proteins. The cell wall is the rigid structure the surrounds the cell and the cell membrane is flexible and skin like and surrounds the cytoplasm.

Explanation:

Hope this helps! pls give brainliest:)

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2 years ago

Explain how selective breeding can lead to some sheep overheating from having too much wool

Snowcat [4.5K]

Answer:

Selective breeding of normal sheep with too much wool sheep.

Explanation:

Selective breeding can lead to some sheep overheating from having too much wool if the sheep of having more wool is crossed with normal wool so the offspring is produced with huge amount of wool on their body that keep its body warm in winter season and overheat in summer season. So the sheep shed their hairs in the summer season in order to reduce overheating of the body.

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2 years ago

Bio what’s the answer?? help

Margaret [11]

It’s the first one!

compact bone is a dense bone with a bony matrix that is solidly filled with arya if ground substance and inorganic salts, leaving only tiny spaces (lacunae) that contain osteocytes, or bone cells. Immature compact bone DOES NOT CONTAIN OSTEONS and has a woven structure.

hope this helps!

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2 years ago