1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.
2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.
3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.
3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.
3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.
3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.
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Answer: increase in MOney Supply
Explanation:
<span>The campaign practice of getting out and speaking to a targeted group of people is called canvassing. Canvassing is a common political campaign strategy where campaigns directly reach out to and contact people to convince them to vote for a certain candidate. This is done by giving a canvasser a list of people to contact by calling voters or knocking on their doors. </span>
prudence, indeed, will dictate that governments long established should not be changed for light and transient causes; and accordingly all experience hath shewn, that mankind are more disposed to suffer, while evils are sufferable, than to right themselves by abolishing the forms to which they are accustomed
In this paragraph, those who were a part in crafting the Independence Declaration display their familiarity with 2d Treatise on Government: Of Civil Government of John Locke. sound judgment of particular circumstances-tells us that people should think long and hard before trying to overthrow any government, however evil.
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Answer:
puffery
Explanation:
The word puffery is used to denote an undue or exaggerated praise. Puffery is a marketing strategy, using which commercial entities advertise their product as best or unique. However, it should be noted that puffery is neither deceptive nor illegal. For example, if one advertises that he makes the best ice-cream, he is not comparing with it others.
