You do not have to wash your hands before putting on gloves, although I do. I have OCD. It's just force of habit. In this case, I would not say true or false because it really depends.There's no rule that says you have to wash your hands before putting on gloves. Although, doctors will meticulously wash their hands before they put on gloves before surgery, even if they are putting on gloves, to make sure no germs travel through the plastic on their behalf and infect the patient internally. But relating to normal checkups, they do not wash their hands before putting on gloves, they just put them on.