Question

(a) Depending on the organism, the optimal pH for enolase to catalyze its reaction is between 6.5 and 8.0. Describe how a pH below or above this range is likely to affect enolase and its catalytic ability.

Answer 1

Answer:

An enzyme can be described as a biological molecule which catalyzes a reaction. Each organism works best at an optimum temperature and pH.

If the pH is too high or too low than the optimum pH, then the enzyme such as enolase will become denatured and it will not be able to catalyze the reaction.

A change in pH causes the enzyme the shape of an enzyme to get changed or it changes the specific charge properties of the substrate of that enzyme. As a result, the substrate is no longer able to fit into the active site of the enzyme. Hence, the enzyme is not able to catalyze the reaction.

Answer 2

Answer:

During gluconeogenesis and glycolysis, Enolase catalyzes the transformation of 2-phosphoglycerate to phosphoenolpyruvate and is needed by every domain of life.

Explanation:

The optimum pH required is 6.5. Changes in pH value affect both hydrogen and ionic which hold the enzymes together. The changes may not only alter the enzyme's structure, but may also modify the substratum's form or charging properties so that either the substratum can't bind to the active site or catalyze it.