Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
Ave you ever wondered why one area of the world is a desert, another a grassland, and another a rainforest? Why are there different forests and deserts, and why are there different types of life in each area? The answer is climate.
<span>Climate is the characteristic condition of the atmosphere near the earth's surface at a certain place on earth. It is the long-term weather of that area (at least 30 years). This includes the region's general pattern of weather conditions, seasons and weather extremes like hurricanes, droughts, or rainy periods. Two of the most important factors determining an area's climate are air temperature and precipitation. </span>
1. The Cell Wall.
Plants have a cell wall in order to maintain rigidity and stability. Animal cells do not have a cell wall because they have a different structure, which makes them more flexible and able to move around easier.
2. Chloroplasts
Since plants cannot move, they get their energy from the sun. This energy is made inside of the chloroplasts. Animal cells don't have chloroplasts because their energy comes from other organisms.
3. Central Vacuole
The central vacuole provides structure and holds materials and wastes, and also decomposes those wastes. Animal cells don't have a central vacuole because they have lysosomes and normal vacuoles that do the same function.
