Due to poor medicine and nutrition most likely
The Silk Road trade allowed for diffusion of technologies to go throughout Eurasia. For instance, silk-making technology from China was able to diffuse and reach areas in Europe. Another example would be the religion of Buddhism. Buddhism originated in India and made its way to China along the Roads, and changed a bit along the way.
Factors leading to the expansion of the Texas frontier. Texas Frontier Timeline, 1866-1875 · Texas Frontier Timeline, 1876-1882 · U.S. Army on the Texas Frontier · Windmills · "Forting up" on the West Texas Frontier.
Answer:
The three aspects of suffering that Buddha described in his famous sermon are the (REALITY) of suffering, the (CAUSE) of suffering, the (ENDING) of suffering, and the way that leads to the end of suffering.