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SashulF [63]
3 years ago
11

Cholesterol auxotrophic cells are unable to produce their own cholesterol so they must rely on LDL present in the cell culture m

edia. Please briefly describe three different ways that can be used to create a cholesterol auxotrophic cell line.

Medicine
2 answers:
arlik [135]3 years ago
6 0

Answer:

Explanation:

See the picture attached

Alenkasestr [34]3 years ago
3 0

Answer:

The three methods are: 1) Using H-Ras- SREBP- 2 Fusion proteins, the NH2 segment can be released by two sequentia

Explanation:

Sterol regulatory element binding proteins (SREBPs) are transcription factors attached to the endoplasmic reticulum. The NH2-segment, which activates transcription, is connected to membranes by a hairpin anchor formed by two transmembrane sequences and a short lumenal loop. Using H-Ras–SREBP-2 fusion proteins, we show that the NH2-segment is released from membranes by two sequential cleavages. The first, regulated by sterols, occurs in the lumenal loop. The second, not regulated by sterols, occurs within the first transmembrane domain. The liberated NH2-segment enters the nucleus and activates genes controlling cholesterol synthesis and uptake. Certain mutant Chinese hamster ovary cells are auxotrophic for cholesterol because they fail to carry out the second cleavage; the NH2-segment remains membrane-bound and transcription is not activated.

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Answer:

The correct answer is B

Explanation:

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3 0
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How might the body respond if it is getting neurotransmitters from an outside source
viva [34]

When the human body receives a neurotransmitter from outside of the body. The first aspect to consider is if the neurotransmitter entered through the blood vessels by injection or by oral intake. When the neurotransmitter is in the blood stream can reach the receptor of this neurotransmitter in the body. If the person is healthy, the receptor will receive an overstimulation so the answer will be stronger than normal quantities of the neurotransmitter. Nevertheless, the reuptake of the neurotransmitter will occur in the same way as natural neurotransmitter.

5 0
3 years ago
What’s the scientific name for a heart attack
marin [14]

Answer:

Myocardial infarction

Explanation:

7 0
3 years ago
Read 2 more answers
There are numerous levels of organization in the body. The
Sholpan [36]

The questions has missed the multiple options. The options are as follows:

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8 0
4 years ago
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The IV order is to infuse Ciprofloxacin 100 mg in 50 mL 0.9% NS q.12h. The pharmacy sends the mixed IVPB with instructions to in
jok3333 [9.3K]

Answer:

<u>Flow rate = 16.67 gtts/min rounded to 17 gtts/min</u>

Explanation:

In order to calculate the flow rate, the following formula will be used.

Flow rate (gtts/min) = \frac{Volume (mL)}{Time (minutes)} Drop factor (gtts/mL)

50 mL of fluid in which Ciprofloxacin is dissolved is taken as volume.

<u>KNOWN DATA:</u>

Volume = 50mL

Time = 45 minutes

Drop factor = 15 gtts/mL

Flow rate = unknown

<u>CALCULATION:</u>

Plugging the known values in the formula,

Flow rate (gtts/min) = \frac{Volume (mL)}{Time (minutes)} Drop factor (gtts/mL)

Flow rate = \frac{50}{45} x 15

<u>Flow rate = 16.67 gtts/min.</u>

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3 years ago
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