1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
SashulF [63]
3 years ago
11

Cholesterol auxotrophic cells are unable to produce their own cholesterol so they must rely on LDL present in the cell culture m

edia. Please briefly describe three different ways that can be used to create a cholesterol auxotrophic cell line.

Medicine
2 answers:
arlik [135]3 years ago
6 0

Answer:

Explanation:

See the picture attached

Alenkasestr [34]3 years ago
3 0

Answer:

The three methods are: 1) Using H-Ras- SREBP- 2 Fusion proteins, the NH2 segment can be released by two sequentia

Explanation:

Sterol regulatory element binding proteins (SREBPs) are transcription factors attached to the endoplasmic reticulum. The NH2-segment, which activates transcription, is connected to membranes by a hairpin anchor formed by two transmembrane sequences and a short lumenal loop. Using H-Ras–SREBP-2 fusion proteins, we show that the NH2-segment is released from membranes by two sequential cleavages. The first, regulated by sterols, occurs in the lumenal loop. The second, not regulated by sterols, occurs within the first transmembrane domain. The liberated NH2-segment enters the nucleus and activates genes controlling cholesterol synthesis and uptake. Certain mutant Chinese hamster ovary cells are auxotrophic for cholesterol because they fail to carry out the second cleavage; the NH2-segment remains membrane-bound and transcription is not activated.

You might be interested in
The pancreas releases alkaline, enzyme-rich pancreatic juice into the ____________ of the small intestine. What is the function
grin007 [14]

Answer:

The correct answer is the duodenum.

Explanation:

Pancreatic juice is an alkaline fluid which contains important enzymes for the digestion of proteins, carbohydrates and fats.  

Pancreatic juice is secreted by the pancreas when food enters the stomach which is released into the "duodenum" of the small intestine. The pancreatic juice enters duodenum through a pancreatic duct which joins with the bile duct coming from the liver and form "ampulla of Vater" in the duodenum.

Pancreatic juice helps in digestion of carbohydrates, proteins and fats as it contains pro-enzymes and enzymes like trypsinogen, chymotrypsinogen, lipase and many more. It also makes the acidic stomach solution alkaline due to the presence of HCO₃.

Thus, the duodenum is the correct answer.

7 0
3 years ago
How long should i take ciprofloxacin 500mg for uti.
Elden [556K]

Answer:

2 times a day for 7-14 days.

Explanation:

5 0
2 years ago
In a human arm, the phalanges are ____________ to the humerus.
Rina8888 [55]
In a human arm, the phalanges are ____________ to the humerus.

Answer: Explanation: Phalanges, the digits of the hand, are the most distal bones of the upper limb. The humerus is the bone of the upper arm. The ulna is the bone of the medial side of the forearm.
7 0
2 years ago
Thyroid hormones are conceptually very similar to steroid hormones.What does this tell you about the mechanism of action in alte
maksim [4K]

Answer:

Explanation:

Their course of action in altering metabolism is very similar because both of them interact with intracellular receptors (primarily the cytosolic receptors present in cytoplasm of the cell) and translocate into the nucleus for performing their desired goal (likely to synthesize a mRNA which can then be turned into a protein to get a desired action going)

7 0
3 years ago
Which of the following tastes is located on the back of the tongue?
Lorico [155]

Answer: Bitter

Explanation:

The taste of Bitterness is the strongest tast located on the back of the tounge.   Sweet is in front, salty and sour is on the sides.  

8 0
3 years ago
Other questions:
  • 7 on the pH scale is considered ____________ where the number of hydroxide (OH) ions is equal to the number of _________ ions.
    5·1 answer
  • *
    13·1 answer
  • What is the mechanism which causes air to enter a person’s lung when inhaling?:
    11·1 answer
  • Explain in detail how educational institutions and personal ideology affect the influence of contemporary
    12·1 answer
  • Do you like my tiny motorcycle
    7·2 answers
  • Tinh dầu nào có vị ngọt
    6·1 answer
  • The hormone that directly controls water reabsorption in the collecting duct of the nephron is.
    10·1 answer
  • A nurse is performing tracheostomy care and suctioning a client who has copious secretions. Which of the following methods shoul
    5·1 answer
  • A client is administered an oral contraceptive. what is the process that occurs between the time the drug enters the body and th
    13·1 answer
  • Why did it take so long to adopt telehealth
    15·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!