Answer:
DNA replication a process of copying of a cell's DNA. DNA replication is semiconservative process which means that each strand in the double helix helps in the synthesis of new, complementary strand and conserve the parent template.
The Molecular mechanism of DNA replication is as following:
- The double starnded DN in binded with hydrogen bond, the enzyme helicase opens up the DNA at the replication fork.
- A single stranded binding protein prevent the rewinding of DNA and so binds to the DNA around the replication fork
- Topoisomerase prevent supercoiling at replication fork.
- The ezymes primase come in action and produces RNA primers which are complementary to the DNA strand.
- DNA polymerase III help to extends the primers and allow them to add to the 3' end, to make new DNA.
- DNA Polymerase then remove RNA primers and replace with DNA.
- DNA ligase blocks the the gaps between DNA fragments.
So, this is the molecuar mechanism of DNA replication.
Answer:
b
Explanation:
its b because it doesnt say any else then 15 or any clues so its tryna trick u
Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Removal of introns and the splicing together of exons.
Introns are regions of the DNA code that do not code for amino acids; therefore, they need to be removed before the mRNA strand is translated. Exons are the parts of the strand that do code for proteins, so they need to be pieced together once the introns are removed.
