A diagnosis is the name or identification of a problem according to ones symptoms
Chinese hamster ovary cell production of recombinant tissue‐type plasminogen activator (t‐PA) was increased by amplification of cotransfected dihydrofolate reeducates cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 μM MTX and yielded 26,000 U/106 cells/day t‐PA (43 μg/106 cells/day). Above 25 μM MTX, cell specific t‐PA production rates became increasingly variable and the cDNA copynumbers decreased. No apparent correlation between the cell specific t‐PA production rate and the growth rate was observed upon sub cloning of the amplified cells. When MTX selection was removed, the t‐PA production rate decreased up to tenfold within 40 days; this was accompanied by an up to 60% drop in cDNA copynumber. Subclones isolated after 108 days of culture in the absence of MTX were, on average, sixfold more stable than their parental cells. In culture without MTX, the maximum stable t‐PA production rate obtained (over 250 days) was 7000 ± 750 U/106cells/day (∼12 μg/106 cells/day), approximately threefold lower than the maximum unstable levels of production reached under selective pressure. Taken together, these results define a wide range of the highest t‐PA expression rates obtained under MTX selection, for which stable expression without selection has not been reported
Answer:
The Answer would be AATGCG.
Explanation: The reason is because A pairs with T and C pairs with G.
Prothallus represents a gametophyte in the life
cycle of fern.
<span>A prothallium, or </span>prothallus<span> (from Latin pro = forwards and Greek θαλλος
(thallos) = twig) is usually the gametophyte stage in the life of a fern or
other pteridophyte. Occasionally the term is also used to describe the young
gametophyte of a liverwort or peat moss as well.</span>
The correct answer between all
the choices given is the second choice or letter B. I am hoping that this
answer has satisfied your query and it will be able to help you in your
endeavor, and if you would like, feel free to ask another question.
