Question

Recall the procedure for measuring tyrosinase activity. what is the purpose of the phosphate buffer

Answer 1

Phosphate buffer is a buffer used in many types of reactions with the function to maintain constant pH value.

This is important because enzymes’ activity is affected by changes in pH. There is an optimum pH for every enzyme that can be defined as most favorable pH value or the point where the enzyme is most active.  Phosphate buffer is often use in the measurement of enzyme’s activity because  it is isotonic and non-toxic to most cells.