Answer:
A
Explanation:
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Reactions that hydrolyze the phosphodiester bonds split the DNA molecule between the phosphate groups and the hydroxyl groups of the two sugar groups.
In DNA there is a covalent bond through a phosphate group that connects the hydroxyl group (OH) at the 5' position of the pentose sugar and the hydroxyl group at the 3' position of the pentose sugar of the next nucleotide. This covalent bond is called a phosphodiester bond because chemically the phosphate group is in the diester form.
In other words, the phosphodiester bond connects the sugar in one nucleotide to the sugar in the next nucleotide, so this bond simultaneously connects the two consecutive nucleotides to form a polynucleotide chain. If there is an enzyme that catalyzes the hydrolysis of covalent bonds that combine nucleotides, what happens is that the phosphodiester link between deoxyribose sugars will break.
Learn more about the phosphodiester bonds at brainly.com/question/23660733
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Discontinuous variable ;)
Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
