Which of the following describes the second law of thermodynamics?

Design an experiment to answer the question, what is the effect of caffeine on the rate of mitosis in frog eggs? Label: hypothes

s2008m [1.1K]

Answer:

Explanation:

Null Hypothesis: Their will be no effect of the caffeine on the mitosis.

Hypothesis: Caffeine will inhibit the SHSY5Y cell division.

Dependent variable: Various concentration of the caffein compound.

Independent Variable: temperatures of the experimental condition. If their will be any effect of the temperature in mitosis then it will also affect the mitosis of control cells.

Experimental design.

Culture SHSY5Y neuronal cell line in the T-75 culture flask in CO2 incubator at 37 degree celsius. Next day, add cells from T-75 flask in the 24 well plate and allow cells to adhere to the well by incubating plate in CO2 incubator at 37 degree celsius. Add the compound caffeine at different concentration (say 1 microgram, 2 microgram, 3 microgram, 4 microgram) and incubate the plate for 24 hour. Determine the mitotic cells by following assay.

Assay to determine mitotic cell.

The reagent succinimidyl ester of carboxyfluorescein diacetate (CFSE) binds to cytoplasmic proteins. it is fluroscent reagent. And when cell divide due to mitosis, cytoplasm will also divide and hence, the protein which stained with the CFSE reagent will also goes in the daughter cell. Then, the fluorescence of the stained cells can be read on the plate reader.

Prepare the graph of the fluorescence values (Y-axis) verses different concentrations of the caffein (X-axis) and determine the slop of curve to know the rate of mitosis.<u> </u>

<u>Following test and control samples will be used for the assay.</u>

1. SHSY5Y cell line with added caffeine. It is test sample.

2. SHSY5Y cell line added with buffer. This important to know behaviour of cells under experimental conditions.

3. SHSY5Y cell line with solvent used to dissolve compound caffeine. It is important to know the toxicity or any effect of solvent on the mitosis.

5 0

2 years ago

Does the net gain of atp in glycolysis differ when glycogen, rather than glucose, is the starting material? what is the change?.

salantis [7]

The energy is greater and producing a net gain of ATP in glycolysis of 3 ATP.

<h3>What is glycolysis?</h3>

The metabolic process known as glycolysis turns the sugar glucose (C6H12O6) into pyruvate (CH3COCO2H). The high-energy molecules adenosine triphosphate (ATP) and reduced nicotinamide adenine dinucleotide are created using the free energy released during this process (NADH).
A series of ten enzyme-catalyzed processes make up glycolysis. binding energy of carbs is captured. Retention of ATP One metabolic route that doesn't require oxygen is glycolysis (In anaerobic conditions pyruvate is converted to lactic acid)
Glycolysis occurs frequently in various species, which suggests that it is an old metabolic route. In fact, the events that make up glycolysis and its companion process, the pentose phosphate pathway, take place in the oxygen-free environment of the Archean oceans, also in the absence of enzymes, and are catalyzed by metal.

To learn more about glycolysis with the given link

brainly.com/question/10554097

#SPJ4

7 0

1 year ago

Blood vessels that absorb strong pressure pulses contain more of this type of tissue.

DochEvi [55]

Blood vessels that absorb strong pressure pulses contain more of elastic<span> tissues. These tissues are bundles of protein that can be found in extracellular matrix of connective tissue. These are produced by fibroblasts and smooth muscle cells found in arteries.
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7 0

2 years ago

Which organism gains energy directly from eating the krill?

Marianna [84]

The answer is whales. In the Antartic and Arctic regions, krills and whales are very abundant in numbers. Whales are number one consumer of krills in these regions. They can feed on a large swarm of krills when they feed. They also have less competition on these icy waters. They gain energy every time from eating them.

6 0

3 years ago

Read 2 more answers

All of the ____ and things ____ in a particular area are called an ecosystem<br> fil in the blanks

andrew11 [14]

Answer:

living, nonliving

Explanation:

all living and non living things in an area make up an ecosystem

8 0

3 years ago