Answer:
Short answer is primers are partially complementary.
Explanation:
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3'
Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3'
When we write reverse primer 5' to 3' we can easily see that
3'-TTGGCCAATGG---5' is complementary to the forward primers'
5'---AACCGGTTACC-3' sequence. So instead of binding to the template DNA these primers might bind each other resulting with reduction of efficiency of DNA amplification.
15-40
Every 1 in 5 sold is caught illegally
Answer:warm weather ,short winters
Explanation:
