Active transport requires energy because unlike osmosis and diffusion which are passive transport methods which sees where the particles are moving along the concentration gradient, in active transport, the particles are moving against the concentration gradient and as such energy through ATP is needed for the particles to be transported.
This affected slaves in a negative way because since the fugitive slaves would be returned to their owner, the slaves would later suffer punishment, beatings, and even death due to them escaping their master. It was a terrible agreement that no state could stop a fugitive slave from being returned to his/her owner.
It would be B, contour plowing
One of the differences is
dna is double stranded
rna is single stranded