Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
Answer:
3:1
Explanation:
i hope it will be helpful
Answer:
True.
Explanation:
The unequal distribution of ions inside and outside of the cell makes the membrane potential of the cell. The potential is important for the conduction of the impulse and generation of the action potential.
The resting membrane potential of the nerve impulse is maintained by the presence of large potassium ions inside the cell. The resting membrane potential is -70mV. The depolarization of the membrane occurs due to influx of the sodium ions.
Thus, the answer is true.
A scientific experiment, in the form of the scientific model, is a testable model used to seek explanation for natural phenomena.