Isocitrate + NAD+ -> a-ketoglutarate + CO2 + H2
The amount of ATP produced from one molecule of glucose will decrease because NADH is no longer produced in the isocitrate dehydrogenase reaction. Without generation of this high energy electron carrier there will be less electrons from one molecule of glucose that are transferred to and through the electron transport chain. So there will be less protons (H+ ions) pumped into the intermembrane space. So there will be less of a H+ ion gradient across the inner mitochondrial membrane to drive the synthesis of ATP by ATP synthase.
The amount of time that the 2 species have been evolving apart??
First you have to change the x value to 4x because for it to be perpendicular it has to be opposite slope so the equation is y=4x-9