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Citrus2011 [14]
3 years ago
14

1. List each of the seven steps involved in rDNA technology, from obtaining large amounts of this gene to confirming your pure p

rotein X gene from an E. coli and describe one of them briefly (2 pts).
Biology
1 answer:
Nastasia [14]3 years ago
3 0

Answer:

Recombinant DNA technology can be described as a technology in which DNA of two different organisms is merged and put into a host. This technology allows the production of genes which are beneficial to humans.

The steps of the rDNA technology are:

1) Isolating of DNA fro a cell

2) Using Restriction Enzymes to cut the DNA

3) Isolation of the desired DNA

4) Amplification of the desired gene

5) Ligating the DNA fragment into a suitable vector

6) Transfer of the foreign DNA into an host organism

7) Getting the gene product

One of the process to describe will be:

Amplification of the desired gene: The most common method of amplifying a gene in vitro is by the technique of PCR.

There are three basic steps of PCR:

Denaturation:

In this step, the two strands of the DNA are separated by heat. Usually the temperature is set to 92-95 degrees  for 15 sec Celsius for this process.

Annealing:

In this step, primers are used to anneal the DNA strands. This step is carried out at lower temperatures mainly in between 60-65 degrees Celsius.

Extension: The Taq polymerase enzyme adds nucleotides to the primers complementary to the DNA strands.

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3 0
2 years ago
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Summary of hearing: Vibrations cause the cochlea’s membrane to shake. This causes ripples in the ____________, bending the _____
mash [69]

Answer:

1) Basilar membrane

2) Stereocilia or hair cells

3) Nerve cells

4) Auditory

5) Temporal lobe

Explanation:

Basilar membrane: located inside of the cochlea which is located in the inner ear. This membrane separates two tubes that is filled with liquid which is also important for hearing.  

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Nerve cells: One of the main cell types in the brain, which are responsible for signal transfer.

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8 0
3 years ago
Any one can please fill all the labellings.<br>thanks:)​
Vika [28.1K]

Answer:

1 = True Ribs or Vertebrosternal Ribs

2 = False Ribs or Vertebrochondral Ribs

3 = Floating Ribs

4 = Thoracic Vertebrae 1 (T1)

5 = Ribs

6 = Coastal Cartilage

Explanation:

Thus, The Whole Structure is known as Rib Cage or Thoracic Cage

<u>-TheUnknown</u><u>S</u><u>cientist</u>

3 0
2 years ago
How does the enzyme that makes rna know where to start transcribing the dna?
Arturiano [62]
The enzyme that makes RNA knows where to start transcribing the DNA by knowing where to start transcribing at the promoter site. For example,<span> TATA Box in Eukaryotes. Hope this answers the question. Have a nice day. Feel free to ask more questions.</span>
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3 years ago
Explain the main functions of the roots
Vesnalui [34]

Answer:

to anchor the plant to the ground. equalizing osmosis in the plant and feeding water up through the roots.

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