Option D, subinfeudation is the right answer.
Subinfeudation in the feudal system, is a practice by which tenants received a land grant from the king or other lords, and by becoming the lord himself he (tenant) gives a part of his granted land to the others. In one way, this practice of subinfeudation can be looked at as a layering of responsibility.
Membranes are primarily composed of a bilayer of lipids (fats).
In addition to lipids, there are proteins, carbohydrates, and combinations of the three within the bilayer, but the barrier that separates the cell from its exterior environment is established by the lipids. It is called a bilayer because, as you can see in the above image, it is formed by sheets of lipids in opposite conformations. How does this occur? By the nature of the lipid - having a polar, hydrophilic head, and a long, hydrophobic. The cells in a sample are separated from each other, often by a physical means such as grinding or vortexing, and put into a solution containing salt. The positively charged sodium ions in the salt help protect the negatively charged phosphate groups that run along the backbone of the DNA.
A detergent is then added. The detergent breaks down the lipids in the cell membrane and nuclei. DNA is released as these membranes are disrupted. To get a clean sample of DNA, it’s necessary to remove as much of the cellular debris as possible. This can be done by a variety of methods. Often a protease ( protein enzyme) is added to degrade DNA-associated proteins and other cellular proteins. Alternatively, some of the cellular debris can be removed by filtering the sample. Finally, ice-cold alcohol (either ethanol or isopropanol) is carefully added to the DNA sample. DNA is soluble in water but insoluble in the presence of salt and alcohol. By gently stirring the alcohol layer with a sterile pipette, a precipitate becomes visible and can be spooled out. If there is lots of DNA, you may see a stringy, white precipitate. The DNA sample can now be further purified (cleaned). It is then resuspended in a slightly alkaline buffer and ready to use.
On June 7, 1494, the governments of Spain and Portugal agreed to the Treaty of Tordesillas, named for the city in Spain in which it was created. The Treaty of Tordesillas neatly divided the “New World” of the Americas between the two superpowers.
Spain and Portugal divided the New World by drawing a line in the Atlantic Ocean, about 370 leagues west of the Cape Verde Islands, then controlled by Portugal. All lands east of that line (about 46 degrees, 37 minutes West) were claimed by Portugal. All lands west of that line were claimed by Spain.
Spain and Portugal adhered to the treaty without major conflict, and the results linger throughout the Americas today. Most Latin American nations are Spanish-speaking countries, for instance, but Portuguese is the leading official language in Brazil. This is because the eastern tip of Brazil penetrates the line agreed to in the Treaty of Tordesillas, so the region was colonized by Portugal.
The treaty ignored any future claims of the British and French, the other European superpowers of the time. The British, French, and Russian empires did not claim parts of the Americas for years after the Treaty of Tordesillas.
Most importantly, however, the Treaty of Tordesillas, completely ignored the millions of people already living in established communities in the Americas. The treaty stipulated that any lands with a “Christian king” would not be colonized. Christianity had not spread to the Americas, and the resulting colonization proved disastrous for indigenous cultures such as the Inca, Taino, Aztec, Tupi, and thousands of other bands throughout the Americas.
The Gutenberg Bible <span>was the first major book printed using mass-produced </span>movable metal type in Europe. It marked the start of the "Gutenberg Revolution<span>" and the age of the printed book in the West. </span>Widely praised for its high aesthetic and artistic qualities,<span>the book has an iconic status.</span>
