Use the numbers to put the steps of translation in order, from first (1) to last (5). The large ribosomal subunit completes the
initiation complex. A stop codon is reached. The initiator tRNA binds to the start codon. Amino acids are paired with anticodons, gradually forming a long chain. The small ribosomal subunit joins the mRNA strand, and the start codon is identified.
Translation is a complex process that translated the genetic information from the language of DNA in the language of RNA. The first step in this process is the binding of the initiator tRNA (that is bound to the aminoacid methionine) to the small ribosomal unit. Then, the small ribosomal unit joins the mRNA; it is the part of the ribosome mainly responsible for translating. After that, the initiator tRNA binds to the start codon. This reaction frees some initiation factors that make large ribosomal units bind to the small one. Hence, <span>the large ribosomal subunit completes the initiation complex afterwards. The role of the large ribosomal unit is mainly to make the peptidic bonds between the aminoacids in the new protein. After that, </span><span>amino acids are paired with anticodons, gradually forming a long chain</span>; this chain is called a polypeptide and is the skeleton of the protein that is created. Finally, this process stops when one of the three possible stop codons are reached.
Diaphorase or dihydrolipoyl dehydrogenase is a flavoprotein enzyme capable of oxidizing the reduced form of NAD (NADH). This lipoamide dehydrogenase is a component of the glycine cleavage system, as well as of the alpha-ketoacid dehydrogenase complexes. It binds one FAD per protein subunit. The enzyme is provided in 3.2 M ammonium sulphate.
