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The coordinating conjuctions in English are just three "and, or, but". 'And' is used to add information, 'or' to indicate alternatives, and 'but' to express contrast. These conjuctions link only units of the same level/ status, for example independent clauses.
1° sentence: Carlos put his books in his backpack, and he walked to the library. Here 'and' is appropiate because you need to add information.
2° sentence: My sister loves dogs but my brother prefers cats. 'But' is the correct one to indicate contrast between the two people's preferences.
3° sentence: Dad will make spaghetti for dinner or he will take us out for pizza. 'Or' is the correct one since the independent sentences signal alternatives
Answer:
it leads readers to wonder what awful deed Great-aunt Gertrude must have. committed
<span>Selective media are used for the growth of only selected microorganisms. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, which do not possess the resistance, from growing. Media lacking an amino acid such as proline in conjunction with E. coli unable to synthesize it were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes.</span>
