The appropriate sequence of steps involved in recombinant DNA technology are as under :-
1) Selected gene is isolated.
2) Restriction enzymes cut DNA into fragments.
3) Fragments of DNA are inserted into a vector.
4) Vector and recombinant DNA multiply.
5) Recombinant DNA is inserted into host.
In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.
In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.
In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.
In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.
In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).
The new Hazard Communication Standards provide teachers and students the right to manipulate chemical hazards.
Chemical hazards are non-biological substances that can cause damage to health. These are used in different enterprises, companies, school, universities and laboratories for different purposes such as cleaning, disinfection, scientific experiments, among others. When handling them, <u>employees are exposed to them and are vulnerable to harm from these chemicals</u>.
There are many types of hazardous chemicals to be wary of, such as:
- Carcinogens
- Neurotoxins
- Acids
- Systemic toxins
- Asmogens
- Silica dust
- Lead
In the workplace, exposure to these substances represents a real occupational hazard and there must be regulations to ensure that they are safe to handle.
The Occupational Safety and Health Administration (OSHA) is the agency of the U.S. Department of Labor which determined the Hazard Communication Standards. <u>This is a document with the necessary information to study the hazards of the chemical substances used by different employees in the companies</u>, so that they are aware of the dangers of these substances. These employees have the right to know exactly what compounds they handle and the risks involved in their use as well as the precautions and care to be taken.
Therefore, by having these safety standards in place in schools and universities, teachers and students have the possibility and the right to handle chemical hazards safely to be able to conduct the class normally and learn what is necessary.
Learn more about chemical hazards here: brainly.com/question/13113515
Answer:
Species name should both be lowercase
Explanation:
According to the rules of binomial nomenclature:
1. The scientific name of an organism is represented by two names- the first genus and the second by its specific epithet.
2. The first alphabet of the genus should be in the uppercase and the first alphabet of the species will be the lowercase.
In the given question the scientific name of humans has been provided as Homo Sapiens in which the first alphabet of the specific epithet is provided in uppercase which must be in the lowercase as "sapiens".
Thus, species names should both be lowercase is correct.
C- the cell is preforming mitosis
The light microscope allowed scientists to see actual cells for the first time.