Write the letter all of the following statements that are NOT true. a. Coding sequences for gene products can be isolated from c
DNA libraries. b. Antibodies are used for Northern blot analysis. c. VNTRs are highly conserved in human populations. d. PCR amplification generates large numbers of linear DNA fragments. e. RNA molecules can be used as hybridization probes in Southern blot analysis. Separate your answer(s) with a comma, and enter only the letter(s) of your choice(s
b. Antibodies are used for Northern blot analysis.
c. VNTRs are highly conserved in human populations.
Explanation:
The northern blot technique is used in molecular biology laboratories for detecting specific RNA molecules in a sample of RNA derived from a particular cell/tissue. Antibodies can be detected by the enzyme-linked immunosorbent assay (ELISA). In ELISA, antigens are used to detect specific antibodies: the addition of the antigen produces a positive reaction i.e., formation of the antigen-antibody binding complex. Subsequently, a second antibody with a particular marker is added to bind with the complex, thereby producing a change in the color and thus evidencing the presence of the target antibody in the sample. On the other hand, the variable number of tandem repeats (VNTR) are short sequences (around 20 to 100 bp) whose number of repetitions varies at specific <em>loci</em> in the genome of many organisms, including humans. Since VNTR sequences exhibit variation in length between individuals of the same species/population, they are widely used as molecular markers to identify individuals, QTL (quantitative trait <em>loci</em>) mapping, establish phylogenetic relationships among individuals/cultivars/lines, etc.
