In alteration of generation means the two forms of life structure interchange as polyp to Medusa or Medusa to polyp
Dilution is necessary during pure culture preparation to reduce the bacterial load and allow readable colonies to grow.
Dilution of bacteria culture is carried out in comparison to McCfarlan standard so as to have a decent number of bacteria that will not be too numerous or too little to observe the growth of distinct colonies during culture preparation.
Without dilution, colonies may form but may be too numerous to count. Too much dilution may also lead to no growth in the culture plates.
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Sexually - better adapt
Reproducing sexually allows for gene variation which helps in changing environments. Asexually would be the same genetically and it would be difficult to adapt