Question

How is the absorption of ultraviolet light by DNA and RNA important in the analysis of nucleic acids? A. Nucleic acids absord UV licht maximaly at wavelengths of 247 to 250 m Using this phenomenon one can oftendermine the presence and concentration of nucleic acids in a mixture, since proteins absorb UV light maximally a 200 m B. Nucleic acids absorb UV light maximaly at wavelengths of 254 to 260 proteins absorb UV maximally at 200 mm m. Using this phenomenon, one can to determine the presence and concentration of nucleic acids in a mixture, since C. Nucleic acids absorb UV light maximalyal Wavelengths of 254 to 280 nm. Using this phenomenon one can hen determine the presence and concentration of nucleic acids in a since proteins absorb UV light maximally at 270 mm D. Nucleic acids absorb UV light maximally at wavelengths of 247 10 250 nm. Using this phenomenon, one can often farmine the presence and concentration of nucleic acids in a mixture, since proteins absorb UV light maximally at 270 nm

Answer 1

Answer:

B. Nucleic acids absorb UV light maximally at wavelengths of 254 to 260. Proteins absorb UV maximally at 200 nm. Using this phenomenon, one can to determine the presence and concentration of nucleic acids in a mixture.

Explanation:

The concentration of a solution of nucleic acid  can be determined by measuring the absorbance at 260 nm, using a  spectrophotometer. An A260 of 1.0 is equivalent to a concentration of 50 μg/mL for double-stranded DNA, or 40 μg/mL for single-stranded DNA or RNA. If the A280 is also determined, the A260/A280  ratio indicates if there are contaminants present, such as residual  phenol or protein. The A260/A280 ratio should be around 1.8 for pure  DNA and 2.0 for pure RNA preparations.