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Ainat [17]
2 years ago
12

How is the absorption of ultraviolet light by DNA and RNA important in the analysis of nucleic acids? A. Nucleic acids absord UV

licht maximaly at wavelengths of 247 to 250 m Using this phenomenon one can oftendermine the presence and concentration of nucleic acids in a mixture, since proteins absorb UV light maximally a 200 m B. Nucleic acids absorb UV light maximaly at wavelengths of 254 to 260 proteins absorb UV maximally at 200 mm m. Using this phenomenon, one can to determine the presence and concentration of nucleic acids in a mixture, since C. Nucleic acids absorb UV light maximalyal Wavelengths of 254 to 280 nm. Using this phenomenon one can hen determine the presence and concentration of nucleic acids in a since proteins absorb UV light maximally at 270 mm D. Nucleic acids absorb UV light maximally at wavelengths of 247 10 250 nm. Using this phenomenon, one can often farmine the presence and concentration of nucleic acids in a mixture, since proteins absorb UV light maximally at 270 nm
Biology
1 answer:
qaws [65]2 years ago
3 0

Answer:

B. Nucleic acids absorb UV light maximally at wavelengths of 254 to 260. Proteins absorb UV maximally at 200 nm. Using this phenomenon, one can to determine the presence and concentration of nucleic acids in a mixture.

Explanation:

The concentration of a solution of nucleic acid  can be determined by measuring the absorbance at 260 nm, using a  spectrophotometer. An A260 of 1.0 is equivalent to a concentration of 50 μg/mL for double-stranded DNA, or 40 μg/mL for single-stranded DNA or RNA. If the A280 is also determined, the A260/A280  ratio indicates if there are contaminants present, such as residual  phenol or protein. <u>The A260/A280 ratio should be around 1.8 for pure  DNA and 2.0 for pure RNA preparations.</u>

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Explanation:

The pattern of gene expression wherein either paternal or maternal gene is expressed in specific cells while the other one is prevented from expression is known as genomic imprinting.

In the given example, the maternal copy of the gene on chromosome 15 is expressed in brain cells while its paternal copy is not expressed in these cells. Hence, the pattern of expression of this gene is regulated through genome imprinting. One of the mechanism is methylation of cytidine residues of CpG islands of the DNA that are more frequently present within promoters of the genes.

When the cytidine residues of these sequences are methylated into 5-methylcytidine, the transcription factors do not bind to these promoters preventing the expression of these genes.

Hence, methylation of cytidine residue in CpG islands of the promoters of the gene present on chromosome 15 could have silenced its expression in brain cells.

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