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Zielflug [23.3K]
3 years ago
10

Which step in the Gram stain is the critical step in differentiating Gram negative cells from Gram positive cells?

Biology
1 answer:
rewona [7]3 years ago
5 0

Answer:

The rapid decolorization with ethanol or acetone

and counterstaining with safranin

When a decolorizer such as alcohol or acetone is added, it interacts with the lipids of the cell membrane. A gram-negative cell loses its outer lipopolysaccharide membrane, and the inner peptidoglycan layer is left exposed: when safranin is added they will turn red. Conversely, gram-positives retain the Cristal violet and will color in purple

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Explain the purposes of gene expression studies. Describe the use of DNA microarray assays and explain how they facilitate such
denis-greek [22]

Answer:

Both gene expression and DNA micro array study about the expression of gene during different stages of development.

Explanation:

The main purpose of gene expression studies is to determine the level of mRNA expressed at different stages of transcription in a tissue or at different stages of cellular development. If a gene is not “ON” during synthesis of RNA and protein, then the desired proteins are not produced. Such studies allow us to turn on such genes.  

DNA microarray  assays easily identify and determine the network of gene expression across the entire genome. The common application of DNA microarray include – mutation analysis and detection, assessment of gene cop, immunoassays etc.

4 0
3 years ago
Which of the following statements about ATP-powered pumps is NOT true? All ATP-powered pumps contain at least one transmembrane
MAVERICK [17]

Answer:

Only P-, F-, and V-class pumps transport ions.

Explanation:

The distinct classes of ATPases include:

1) Only the P-type ATPase actively transports ions across biological membranes. P-ATPases (also named E1-E2 ATPases) are found both in plasma and organelle membranes. These ATPases serve to transport ions and phospholipids by hydrolyzing ATP to ADP and phosphate.

2) A- and F-ATPases synthesize ATP by transforming the energy from a gradient of ions across the cell membrane.

3) V-ATPase (also known as Vacuolar-H+ ATPases) acidifies vacuole, lysosome, endosome and Golgi membranes. This type of ATPase couples the hydrolysis of ATP to the active transport of protons across biological membranes.

4) E-ATPases hydrolyze extracellular ATP.

5 0
3 years ago
Which of these best describes what occurs during cytokinesis?
larisa86 [58]
Cytokinesis occurs after mitosis is complete. It is when two daughter cells are formed, each with their own nuclei, after a single cell has <em>completely </em>divided.
3 0
3 years ago
Read 2 more answers
Identify which two of Koch's postulates would most likely not be fulfilled when trying to link a human virus to a particular dis
hammer [34]

Answer:

 A) & B)

Explanation:

A) There are certain viruses that are present in both healthy and disease people.

Example: Polio virus is found in many human but it causes disesease in only 1% of human.

B) Viruses require host machinery for replication so they may not replicate in pure culture.

The postulate C is also violated, the virus may not produce disease in non-human experimental host because of inappropriate animal or vaccinated animal etc. Others exception includes, the same disease may be caused by various organisms and different organisms can also cause the same disease.

3 0
2 years ago
Four stages of mitotic cell division are shown in the diagram. ( See screenshot below )
Diano4ka-milaya [45]

The correct answer is C.  The separation of chromatids happens during Anaphase, which is represented by R in the diagram.

4 0
3 years ago
Read 2 more answers
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