The last battle is the answer :)
The Holy Roman Empire and the church were, as you might guess based on the name, quite connected entities in the beginning. The church was responsible for the crowning of Charlemagne, the first emperor who formed the actual Holy Roman Empire. However, in the centuries that followed, one of the emperors of the Holy Roman Empire got into an argument with the pope of the time because of which important consequences ensued.
1. Missouri Compromise (allowed slavery in Missouri)
2. Kansas-Nebraska Act (made Kansas a territory free do decide on slavery)
3. Dred Scott (made black people property)
4. Fugitive Slave Act (forced north to cooperate with slave owners)
5. Bleeding Kansas (Missouri pro-slavery men invaded and terrorized Kansas to force slavery)
Missouri compromise was the beginning were it all started.
Answer:
A:A ban on settlement west of Appalachian mountains caused colonial discontent.
Explanation:
Hopefully this helps