Answer:
CCAGGCC
CCATCGA
GGCCATC
CAT
AGGCCAT
CATCGAG
Explanation:
Shotgun sequencing is a method used to determine the nucleotide sequence of entire chromosomes/genomes. This sequencing method consists of obtaining random DNA fragments which are subsequently classified by bioinformatic tools that ordering them according to overlapping sequences called contigs. In the whole-genome shotgun (WGS) technique, the entire genome of an organism is sequenced, being the critical factor the depth of sequencing, which refers to the quality of the sequencing reads (e.g., a depth of 20X indicates that the genome is sequenced 20 times by a sequencing machine). For the human genome, WGS became available after the completion of the Human Genome Project (HGP), which enabled the generation of a reference sequence for the whole human genome. The steps of the WGS technique are the following:
1. Preparation of isolated chromosomes
2. The DNA is sheared into small fragments
3. The DNA fragments of about 1 kilobase (1000 base pairs) are incorporated into plasmids which are cloned to render pure samples of each DNA fragment
4. The plasmid clones are sequenced by sequencing machines
5. Bioinformatic tools finally are used to link DNA fragments by their overlapping ends
<span>Every living thing needs food. Whether it makes it, or gets it from another living thing by eating that living thing. No living thing can survive without a food source. Food is where you get the energy for life.</span>
Well I'm not exactly certain where the teacher is going with this, but an often used example is red blood cells (RBCs) aka: erythrocytes.
RBCs are suspended in blood plasma as they flood through vessels around and around the body, so the osmolarity (amount of small particles that affect osmosis) must remain relatively constant. This is termed "isotonic", meaning the same amount of osmosis-influencing particles that are there inside the RBCs' cytosol, within their plasma membranes.
If the plasma osmolarity get too high, called hypertonic (as with extra salt particles) then water inside the RBCs will have an osmotic force driving it out of the cells' membranes, to flow where there are more salt particles. This will lead to cell shrinkage (called "crenation").
Counter to that, if the plasma osmolarity gets too low, as due to low plasma salt with excessive water intake (for example from the condition "water intoxication"), then the plasma will be hypotonic with respect to the intracellular cytosol concentration. This can result in water rushing into the RBCs' membranes via osmosis, causing the cells to swell from discs into spheres (balls), or even rupture and burst (a phenomenon called "hemolysis").
HOPE THOSE EXAMPLES HELP!!
The main difference between prokaryotes and eukaryotes is the presence of nucleus in eukaryotes and its absence in prokaryotes. Also, prokaryotes are unicellular, so one cell is a whole organism. On the other hand, eukaryotes are mainly multicellular, and thus more complex. More complex DNA is inside the nucleus in the eukaryotes which enables them to conduct a wider range of functions. Prokaryotes, as a single cell, have simple functions present.
They are absorbed in the small intestine, but I am not 100% sure if it is in the beginning or the middle.
