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Ivanshal [37]
3 years ago
13

Please 30 points!!!!!

Biology
2 answers:
Lisa [10]3 years ago
8 0

Answer:

I love how u said 30 points but it is actually 15

Explanation:

Vlad1618 [11]3 years ago
3 0

Answer:

I think 2 is right

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One of your lab partners has followed the recommended procedure of running Gram-positive and Gram-negative control organisms on
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Reduced in holding time of decolrization step and also used less Alcohol because decolrization step is important in Gram's staining.The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative..

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Gram' staining is a technique used in microbiology labs to differentiate between Gram's positive and negative

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<u>:Gram-negative bacteria </u>Stain red or pink due to retaining the counter staining dye called Safranin or neutral red.

There are four basic step in Gram" staining

1) Application of the Primary Stain to a Heat-Fixed Smear of Bacterial Culture

2)Addition of Gram's Iodine

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The decolorization step must be performed carefully. Otherwise over-decolorization may occur. This step is critical and must be timed correctly otherwise the CV stain will be removed from the Gram-positive cells. If the decolorizing agent is applied on the cell for too long time, the Gram-positive organisms to appear Gram-negative. Under-decolorization occurs when the alcohol is not left on long enough to wash out the CV-I complex from the Gram-negative cells, resulting in Gram-negative bacteria to appear Gram-positive.

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