Answer:
Tris-HCl (0.5M Tris Base, pH7.6):
Trizma Base ---------------------------------- 61 g
Distilled water ------------------------------- 1000 ml
Adjust pH 7.6 using concentrated HCl
Store this solution at room temperature. Dilute 1:10 with distilled water before use
and adjust pH if necessary.
20X Tris-HCl (1M Tris Base, pH7.6):
Trizma Base ---------------------------------- 122 g
Distilled water ------------------------------- 1000 ml
Adjust pH 7.6 using concentrated HCl
Store this solution at room temperature. Dilute 1:20 with distilled water before use
and adjust pH if necessary.
10X Tris-HCl-Tween 20 (0.5M Tris Base, 0.5% Tween 20, pH7.6):
Trizma Base ---------------------------------- 61 g
Distilled water ------------------------------- 1000 ml
Adjust pH 7.6 using concentrated HCl and then add 5 ml of Tween 20.
Store this solution at room temperature. Dilute 1:10 with distilled water before use
and adjust pH if necessary.
Note: Tris-HCl Buffer is used for specific cases of immunohistochemical staining.
*** OR you can use Tris Base to make Tr
