To test for the keystone species hypothesis, the wolves in another region can be removed or trees can be reintroduced to see the effect on the ecosystem.
<h3>What is a keystone species?</h3>
A keystone species is an organism whose existence defines an entire ecosystem such that them, the ecosystem would be severely affected or may not exist.
Trophic cascades are events which result in serious distortions in an ecosystem as a result of the removal or addition of a top predator.
To test for the hypothesis of keystone species and trophic cascades, the wolves in another region can be removed or trees can be reintroduced to see the effect on the ecosystem.
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The vector, which is often a small, circular piece of dna that can exist outside the bacterial chromosome, is known as a plasmid.
Bacteria and certain other microscopic species contain plasmids, which are tiny circular DNA molecules. Physically distinct from chromosomal DNA, plasmids multiply on their own. They normally contain only a few genes, including some linked to antibiotic resistance, and they can spread from one cell to another.
Recombinant DNA techniques are used by scientists to splice the genes they want to research into a plasmid. The inserted gene is duplicated along with the plasmid when it duplicates itself. Molecular cloning, the process of creating DNA molecules and introducing them into a host cell, uses plasmid vectors as the means of delivering recombinant DNA into the host cell.
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<h2>cAMP and glucose mobilization</h2>
Explanation:
It would maintain high cAMP level and elevate glucose mobilization
- Phosphodiesterase is an effector enzyme which degrades secondary messenger cAMP(cyclic adenosine monophosphate)
- Here in this case an inhibitor is inhibiting the phosphodiesterase therefore cAMP level will increase
- As cAMP level rise it activates a protein called protein kinase A which phosphorylates phosphorylase kinase and activates it
- Phosphorylase kinase becomes active that phosphorylates glycogen phosphorylase and makes it active,glycogen phosphorylase catalyse breakdown of glycogen(in liver and muscle cells)
- In liver cells breakdown of glycogen occurs and glucose 1 phosphate gets converted into glucose and supplied to whole body through blood
