After crystal violet staining, the Gram-positive bacteria may appear dark blue or violet. The cell wall of gram positive bacteria have thick peptidoglycan layer, thus it retains the crystal violet stain compared to gram negative bacteria.
Chromosomal deletion is the loss of genetic code, caused by a segment of chromosome breaking away during DNA replication. The best answer is the third one, "when part of a chromosome breaks off and does not reattach."
This is a process called transcription and translation.
Information to synthesize a particular protein is found in DNA in the cell nucleus. This information is copied (transcribed) onto messenger RNA or mRNA in short. The copying process is called transcription.
mRNA then leaves the nucleus and enters the cytoplasm where it attaches to a ribosome. Transfer RNA or tRNA then begins to read (translate) the information on the attached mRNA. This is the process of translation.
tRNA then fetches amino acids that correspond to this information and brings them to the ribosome where they are linked together into a chain. This chain of amino acids is the primary structure of the protein.