Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
It is clinically used to separate <span>a mixed population of macromolecules such as DNA or proteins in a matrix of </span>agarose<span>, which is one of the two main components of agar.</span>
True, you cant always have the ideal conditions in the real world
Answer: The mesosphere starts at 31 miles (50 km) and extends to 53 miles (85 km) high. The top of the mesosphere, called the mesopause, is the coldest part of Earth's atmosphere, with temperatures averaging about minus 130 degrees F (minus 90 C). This layer is hard to study
Explanation: hope this helps please give brainliest!
Answer:
An egg placed in salt water will float!
Explanation:
In normal tap water, an egg will sink because it's density is greater than the density of water. But, when salt is added to the water, it's density becomes greater than the egg's density. The term used to describe this is "buoyancy."
