The nucleotide sequence would have to have 900 nucleotides in order to code for a protein of 300 amino acids. When translating RNA into proteins, RNA is read 3 bases at a time. Each group of 3 bases is a codon, and each codon codes for an amino acid. When read, the proper amino acid is added to a growing chain of amino acids, which will be folded to become a protein.
Therefore, 300 amino acids * 3 nucleotides per amino acid = 900 nucleotides.
Answer:
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Answer:
DNA from a gene of interest can be inserted into a plasmid, then the modified plasmid can be inserted into a bacterial cell to replicate a gene of interest many times.
Explanation:
Plasmids are the extra-chromosomal circular DNA present in bacterial cells. Plasmids are able to replicate themselves independent of genetic DNA. Their ability to self replicate allows them to maintain themselves in the bacterial cells. This is why plasmids are used as cloning vectors in recombinant DNA technology.
A gene of interest is isolated from the donor cell and is inserted into the plasmid. The recombinant plasmid is introduced into bacterial cells where it replicates the ligated desired gene and allows the gene cloning. For example, the human insulin gene is ligated with plasmid and the recombinant plasmid is introduced in <em>E. coli</em> where it replicates the human insulin gene and allows the production of desired copies of the gene.
Yes. With the help of every individual organelle in the cell's body, a cell can keep itself alive. For example, they can use cellular respiration to create ATP.
