Answer:
b. Forward or reverse primers
Explanation:
Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.
Answer:
A possible hypothesis would be:
IF a yeast cell is supplied with maltose sugar, THEN it would yield the greatest amount of energy when metabolized by bacteria
Explanation:
The hypothesis is a testable explanation aimed at providing a theoretical explanation to a question. The hypothesis is a predictive statement about the possible solution, which can be tested. A hypothesis must be testable via experimentation. It often has an IF, THEN format.
Hence, in the case experiment where a student wanted to know which sugar (maltose, glucose or sucrose) would yield the greatest amount of energy for the cell when metabolized by mitochondria. A possible hypothesis of this experiment will be that: IF a yeast cell is supplied with maltose sugar, THEN it would yield the greatest amount of energy when metabolized by bacteria
Note that, this hypothesis must be testable by conducting an experiment and it doesn't have to be true. It can be disproved or proved by the outcome of the experiment. In this case, the hypothesis is giving an educated guess that maltose is the sugar that will yield the greatest amount of energy for yeast cells
