Question

Several examples of antisense RNA regulating translation in bacterial cells have been discovered. Molecular geneticists have also used antisense RNA to artificially control transcription in both bacterial and eukaryotic genes. To inhibit the transcription of a bacterial gene with antisense RNA, what sequence might the antisense RNA contain

Answer 1

Answer:

An antisense sequence with complementary to the target mRNA encoded by the gene of interest

Explanation:

An antisense sequence is usually a short RNA sequence specifically designed in the laboratory to bind by complementary to the DNA/RNA sequence of interest (usually a target mRNA) whose expression is desired to be inhibited. When an antisense bind to its target mRNA, the antisense sequence acts to block translation, thereby inhibiting protein synthesis. Moreover, it has been shown that the double-stranded antisense-RNA complexes may also increase the susceptibility of the target sequence to RNase H digestion.