Answer: The bacteria transformed with this particular plasmid will form white colonies on the plates containing ampicillin and Xgal.
Explanation: The lacZ gene produces an enzyme called β-galactosidase which is responsible for the breakdown of lactose into glucose and galactose. The lacZ gene is one of the three genes (the other two being lacA and lacY) of the lac operon which is responsible for the transport and mechanism of lactose in E. coli and many other bacteria.
In recombinant DNA technology, when a plasmid is to be used to transform a host cell, such markers are used to help screen the transformed cells from the ones that have not taken up the plasmid. Xgal present in the plates is an artificial substrate which is hydrolyzed by
β-galactosidase into 5-bromo-4-chloro-indoxyl which will dimerize and oxidise into 5,5'-dibromo-4,4'dichloro-indigo. This is a blue pigment which will give blue color to the bacterial cells. Introducing a DNA fragment in this lacZ gene will make it non-functional so it will not be able to produce the enzyme.
Therefore, when a bacterial cell is transformed with a plasmid containing ampicillin resistance gene and a DNA fragment introduced in the lacZ gene and then grown on plates containing ampicillin and Xgal, white colored colonies will appear. The white colonies will show the bacterial cells that have successfully taken up the plasmid with the DNA fragment incorporated in the lacZ gene as this will render the gene non-functional and will not produce β-galactosidase which will breakdown Xgal to give blue colonies. Since the plates contain ampicillin, only the bacterial cells that have been successfully transformed with the plasmid ( the ones that have the DNA fragment and the ones without it) will grow as the ampicillin resistance will give them resistance against ampicillin in the plates. The bacterial cells that have not taken up the plasmid will not be resistant to ampicillin and will not form colonies on the plate.
This is called blue-white screening which is used to identify successfully transformed host cells. A picture of this is given in the attachment, taken from the following website:
https://www.mun.ca/biology/scarr/Blue_&_White_Colonies.html
Answer: The body uses carbohydrates for both long term and short term energy storage
Explanation: Some of the energy our body saves is only there for a certain amount of time or we use our energy so therefore it is Short term storage
We also have long term storage since we don't use all of our energy all the time and it needs to be stored some where
#1: Bacteria are like eukaryotic cells in that they have cytoplasm, ribosomes, and a plasma membrane. Features that distinguish a bacterial cell from a eukaryotic cell include the circular DNA of the nucleoid, the lack of membrane-bound organelles, the cell wall of peptidoglycan, and flagella. #2: Archaea have more complex RNA polymerases than Bacteria, similar to Eucarya. Unlike bacteria, archaea cell walls do not contain peptidoglycan. Archaea have different membrane lipid bonding from bacteria and eukarya. There are genetic differences. #10: Bacteria are classified into 5 groups according to their basic shapes: spherical (cocci), rod (bacilli), spiral (spirilla), comma (vibrios) or corkscrew (spirochaetes). They can exist as single cells, in pairs, chains or clusters. #12: Bacteria reproduce .In this process the bacterium, which is a single cell, divides into two identical daughter cells. Binary fission begins when the DNA of the bacterium divides into two (replicates). Each daughter cell is a clone of the parent cell. #13: Pathogenic bacteria are bacteria that can cause disease. ... One of the bacterial diseases with the highest disease burden is tuberculosis, caused by the bacterium Mycobacterium tuberculosis, which kills about 2 million people a year, mostly in sub-Saharan Africa. Infection with a pathogen does not necessarily lead to disease. Infection occurs when viruses, bacteria, or other microbes enter your body and begin to multiply.Pathogenic microbes challenge the immune system in many ways. Viruses make us sick by killing cells or disrupting cell function. #14: Antibiotics work by affecting things that bacterial cells have but human cells don't. For example, human cells do not have cell walls, while many types of bacteria do. The antibiotic penicillin works by keeping a bacterium from building a cell wall. HOPE I HELPED I Don’t NO #11
Answer:
The independent variables is Texting speed in words.
Dependent variable is minutes.
Explanation:
Independent variable is a variable that can stand on it own and does not depend on other variable to change.
Minutes is independent variable, it can stand in it's own.
Dependent variable is a variable that depend on another variable. Texting speed is dependent variable because it's depend on time to change or stand.
Answer:
Pressure with Height: pressure decreases with increasing altitude. The pressure at any level in the atmosphere may be interpreted as the total weight of the air above a unit area at any elevation. At higher elevations, there are fewer air molecules above a given surface than a similar surface at lower levels.In addition, gravity is weaker at the equator due to centrifugal forces produced by the planet's rotation. It's also weaker at higher altitudes, further from Earth's centre, such as at the summit of Mount Everest
