Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
1 is the answer because it’s talking about chemical
Answer:
<h2>A
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Explanation:
1. The cell is present in some type of cells. It is found in plants and bacteria and some others. It is present or found outside the plasma membrane.
2. The functions of cell wall are that it provides tensile strength and protection to the cell against many type of stress. It also plays very important role in many others protections for the cell. There are two layer, named as primary cell wall and secondary cell wall.
3. Composition of Plant cell walls is that they are primarily made of cellulose. Cellulose fibers are linear long polymers of around 100 molecules of glucose. These fibers cluster into bundles, known as or called microfibrils. In plants the microtubule cytoskeleton are present, and there function is that they directs the orientation of cell wall as in which orientation cellulose is deposited in the cell wall.
Answer:
Thus, the correct answer is 'Carbon dioxide, water, and sunlight'.