1. Our results showed that test tube B contained end products of protein digestion, meaning that the isolated enzyme has the ability to break down protein. The end products of protein digestion include small polypeptides, dipeptides, and amino acids. In general, test tube B will contain the building blocks of proteins, which are the amino acids.
2. The two test tubes were placed in a hot water bath at 37oC. This is because the enzyme studied is a human enzyme and it works best at the human body temperature, which is approximately 37oC.
Enzymes are biological catalysts, which means that they accelerate chemical reactions. As a result, an enzyme's activity is greatly affected by temperature. Every enzyme, depending on each function and origin, has a specific range of ideal temperature conditions. If the temperature rises above the maximum accepted, then the enzyme denatures and loses its structure.
3. If the enzyme studied was added in a tube containing starch instead of proteins, then nothing would happen. This is because the enzymes are highly specialized and have very specific actions. It has been proved that this enzyme's activity involves the digestion of proteins.
Starch is a polysaccharide produced by plants, this means that starch is a carbohydrate. Given that starch is not a protein, it will not be digested by the enzyme. An enzyme digesting proteins, will not have any effect on carbohydrates.
4. The smaller molecules which are the result of the digestion happening in the digestive system are absorbed by the human body. More specifically, the products of protein digestion are absorbed by the intestine and then transported into the bloodstream. From the blood, they are then absorbed by other tissues.
After they are absorbed, they are used to build muscles or other compounds which can produce energy.
mRNA will serve as reference book that contains information as the DNA and its sequence is complementary to the DNA template.
The transfer of information in a DNA strand to a new molecule
of messenger RNA is known as transcription. Thus, the process of transcription
is carried out by an enzyme called RNA polymerase and a number of accessory
proteins called transcription factors. However, DNA is copied into mRNA because mRNA will serve as
reference book that contains information as the DNA and its sequence is
complementary to the DNA template.
An invasive species is a species that is not native to a specific location, and that has a tendency to spread to a degree believed to cause damage to the environment, human economy or human health.
Genetic material is found in Eukaryotic cells. Plant and animal cells both are eukaryotic cells.
Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
