Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Answer:
the pheno type is the blue eyes whilst the genotype could possibly be for example AA or Aa. The phenotypes for the parents would be either AA or Aa
Explanation:
A - Ff and Ff is the answer
The correct answer is ANTHROPOLOGIST.
An anthropologist is a professional, who engaged in the study of various aspects of human within past and present societies. Such professionals are involved in the research and study of socio-historical, linguistic, and biological aspects of human. Biologist, chemist and botanist are actively involved in the development of drugs.<span />
Explanation:
ATP is the short form of Adenosine Triphosphate. The molecule is made up of three basic things:
- Adenine ring
- Ribose sugar
- Three phosphate groups(triphosphate)
This molecule is provides the energy needed for life processes when broken down.
The elements that makes up ATP are:
- Carbon(C)
- Hydrogen(H)
- Nitrogen(N)
- Oxygen(O)
- Phosphorus(P)
Learn more:
ATP brainly.com/question/4957918
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