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There are 27 x 10⁷ cells in total for the given conditions of cell culture.
Since it is given that the cell culture contains 3 x 10⁵ cells per ml and there are 12 culture flasks in total. It is also given that each of the culture flask holds 75 ml of culture.
Therefore, to calculate the total capacity of the flasks the total number of flasks should be multiplied with the individual capacity of a flask:-
75 x 12 = 900 ml
Now, in order to calculate the total number of cells in the given culture., the total capacity of the flasks i.e. 900 ml should be multiplied with 3 x 10⁵ cells per ml in the following manner:-
900 x (3 x 10⁵) = 2700 x 10⁵ = 27 x 10⁷ cells
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Answer
I added 4 and 5 as an attachment due to time constraint.
Explanation:
1. at a steady state ca2+ taken by vesicle = 40n/mol
enclosed volume of CSR = 5μ/mg
concentration of ca2+ in vesicle =
40*10⁻⁶/5x10⁻⁶
= 0.008
= 8x10⁻⁹mol/L
2. radius of circle =75nM
volume = 150nm
volume of vesicle = 4/3 x pi x r³
= 1.33 x 3.14 x (75x10⁻⁹)³
= 1.77 x 10⁻²¹
when we convert this we get
1.77x10⁻¹⁸L since 1m³ is equal to 100oL
surface area =4x3.14x(75x10⁻⁹)²
= 12.56x5.625⁻¹⁵
= 7.065x10⁻¹⁴m²
this is 7.065x10⁻¹⁰cm²
3. number of vesicle/CSR
= 5x10⁻⁶/1.77x10⁻¹⁸
= 2.824x10¹²
surface are = 2.824x10¹² x7.06x10⁻¹⁰
= 19.95x10²
I believe you’re referring to the body’s fight or flight response.